Soil contamination by petroleum hydrocarbons constitutes a considerable environmental risk due to their toxicity. In recent decades, several biological and chemical technologies have been developed for remediating in situ soils and waters affected by leakages of diesel fuel. The aim of this study is to assess the soil CO2 efflux as an early measuring tool of the effectiveness of these remediation treatments applied in situ on diesel polluted soils. The study site was located in a tidal salt marsh ecosystem in the Cádiz Bay, where two zones were distinguished according to the level of diesel pollutant (high-polluted and low-polluted areas). In the high-polluted area, three remediation technologies (phytoremediation, bioremediation, and chemical oxidation) were applied individually as well as in combination in order to identify synergies that improve the decontamination performance. The specific objectives of the study were (1) to determine soil CO2 efflux in a diesel polluted tidal salt marsh under a Mediterranean climate; (2) to examine the relationships between soil moisture content, temperature and soil CO2 efflux; (3) to test whether the different remediation treatments promote an early response in soil CO2 efflux. The initial results showed a positive correlation between soil temperature fluctuations and soil CO2 efflux in the low-polluted area of the marsh, but no significant relationships were detected in the high-polluted area. On average, remediation treatments lead to greater soil CO2 efflux rates (81.3 and 294.8 mg CO2-C m-2 h-1 before and after treatment implementations respectively). Of all the remediation treatments, only those plots in which pure biological treatments were employed (phytobarrier, phytoremediation and bioremediation) displayed a clear early response in soil CO2 efflux.
Almagro M, López J, Querejeta JI, Martínez-Mena M. 2009. Temperature dependence of soil CO2 efflux is strongly modulated by seasonal patterns of moisture availability in a Mediterranean ecosystem. Soil Biol Biochem. 41:594-605.
Beames A, Broekx S, Heijungs R, Lookman R, Boonen K, Van Geert Y, Dendoncker K, Seuntjens P. 2015. Accounting for land-use efficiency and temporal variations between brownfield remediation alternatives in life-cycle assessment. J Clean Prod. 101:109-117.
Bento FM, Camargo FAO, Okeke BC, Frankenberger WT. 2005. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresour Technol. 96:1049-1055.
Cadotte M, Deschênes L, Samson R. 2007. Selection of a remediation scenario for a diesel-contaminated site using LCA. Int J Life Cycle Assess. 12:239-251.
Cai WJ. 2011. Estuarine and Coastal Ocean Carbon Paradox: CO2 Sinks or Sites of Terrestrial Carbon Incineration? Ann Rev Mar Sci. 3:123-145.
Camenzuli D, Freidman BL. 2015. On-site and in situ remediation technologies applicable to petroleum hydrocarbon contaminated sites in the Antarctic and Arctic. Polar Res. 34:24492.
Chang W, Dyen M, Spagnuolo L, Simon P, Whyte L, Ghoshal S. 2010. Biodegradation of semi- and non-volatile petroleum hydrocarbons in aged, contaminated soils from a sub-Arctic site: Laboratory pilot-scale experiments at site temperatures. Chemosphere 80:319-326.
Chen M, Xu P, Zeng G, Yang C, Huang D, Zhang J. 2015. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs. Biotechnol Adv. 33:745-755.
Chirwa E, Bezza F. 2015. Petroleum Hydrocarbon Spills in the Environment and Abundance of Microbial Community Capable of Biosurfactant Production. J Pet Env Biotechnol 6:1-5.
Díaz-Puente FJ, Pindado O, Nande M, Fajardo C, Reino S, Rodríguez-Rastrero M, Escolano O, Millán R, Martín M. 2015. Microbial population dinamic during ISCO treatment processes. Laboratory assays and field monitoring. In: Kalogerakis N, Fava F, Manousaki E, editors. Book of Abstracts of the 6th European Bioremediation Conference. Greece: Grafima Publ. p. 43.
Fang C, Moncrieff JB. 2001. The dependence of soil CO2 efflux on temperature. Soil Biol Biochem. 33:155-165.
Fox J, Weisber S. 2011. An R Companion to Applied Regression. 2nd edition. Thousand Oaks, California: SAGE Publications, Inc. 472 p.
Frankignoulle M. 1998. Carbon Dioxide Emission from European Estuaries. Science 282:434-436.
Fuentes S, Méndez V, Aguila P, Seeger M. 2014. Bioremediation of petroleum hydrocarbons: Catabolic genes, microbial communities, and applications. Appl Microbiol Biotechnol. 98:4781-4794.
Gallego JLR, Loredo J, Llamas JF, Vázquez F, Sánchez J. 2001. Bioremediation of diesel-contaminated soils: Evaluation of potential in situ techniques by study of bacterial degradation. Biodegradation 12:325-335.
Gao YC, Guo S-H, Wang JN, Li D, Wang H, Zeng DH. 2014. Effects of different remediation treatments on crude oil contaminated saline soil. Chemosphere 117:486-93.
Guo HQ, Noormets A, Zhao B, Chen JKQJ, Sun G, Gu YJ, Li B, Chen JKQJ. 2009. Tidal effects on net ecosystem exchange of carbon in an estuarine wetland. Agric For Meteorol. 149:1820-1828.
Högberg P, Nordgren A, Buchmann N, Taylor AF, Ekblad A, Högberg MN, Nyberg G, Ottosson-Löfvenius M, Read DJ. 2001. Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature 411:789-92.
Huguenot D, Mousset E, van Hullebusch ED, Oturan MA. 2015. Combination of surfactant enhanced soil washing and electro-Fenton process for the treatment of soils contaminated by petroleum hydrocarbons. J Environ Manage. 153:40-47.
Jiménez OP, Pastor RMP, Segovia OE. 2014. An analytical method for quantifying petroleum hydrocarbon fractions in soils, and its associated uncertainties. Anal Methods 6:5527-5536.
Khan S, Afzal M, Iqbal S, Khan QM. 2013. Plant-bacteria partnerships for the remediation of hydrocarbon contaminated soils. Chemosphere 90:1317-32.
Lai C, Huang Yc, Wei Y, Chang J. 2009. Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil. J Hazard Mater. 167:609-614.
Lemming G, Chambon JC, Binning PJ, Bjerg PL. 2012. Is there an environmental benefit from remediation of a contaminated site? Combined assessments of the risk reduction and life cycle impact of remediation. J Environ Manage. 112:392-403.
Li X, Wang X, Ren ZJ, Zhang Y, Li N, Zhou Q. 2015. Sand amendment enhances bioelectrochemical remediation of petroleum hydrocarbon contaminated soil. Chemosphere 141:62-70.
Lijzen J, Baars A, Otte P, Rikken M, Swartjes F, Verbruggen E, van Wezel A. 2001. Technical evaluation of the intervention values for soil/sediment and Groundwater. Human and ecotoxicological risk assessment and derivation of risk limits for soil, aquatic sediment and groundwater. Report 711701023. Bilthoven, The Netherlands: RIVM, Rijksinstituut voor Volksgezondheid en Milieu.
Majone M, Verdini R, Aulenta F, Rossetti S, Tandoi V, Kalogerakis N, Agathos S, Puig S, Zanaroli G, Fava F. 2015. In situ groundwater and sediment bioremediation: barriers and perspectives at European contaminated sites. N Biotechnol. 32:133-146.
Mater L, Rosa EVC, Berto J, Corrêa AXR, Schwingel PR, Radetski CM. 2007. A simple methodology to evaluate influence of H2O2 and Fe2+ concentrations on the mineralization and biodegradability of organic compounds in water and soil contaminated with crude petroleum. J Hazard Mater. 149:379-386.
Parkin TB, Venterea RT, Hargreaves SK. 2012. Calculating the Detection Limits of Chamber-based Soil Greenhouse Gas Flux Measurements. J Environ Qual. 41:705-715.
Pinedo J, Ibáñez R, Lijzen JP A, Irabien Á. 2013. Assessment of soil pollution based on total petroleum hydrocarbons and individual oil substances. J Environ Manage. 130:72-79.
Presidency Ministry. 2005. Royal Decree 9/2005 of 14 January which establishes a list of potentially soil contaminating activities and criteria and standards for declaring that sites are contaminated. Official State Bulletin 15/2005. Madrid, Spain. p. 1833-1843.
Qi Y, Xu M. 2001. Separating the effects of moisture and temperature on soil CO2 efflux in a coniferous forest in the Sierra Nevada mountains. Plant Soil 237:15-23.
R Development Core Team. 2014. R: A Language and Environment for Statistical Computing. Vienna, Austria: The R Foundation for Statistical Computing.
Raich JW, Tufekciogul A. 2000. Vegetation and soil respiration: Correlations and controls. Biogeochemistry 48:71-90.
Rayment M, Jarvis P. 2000. Temporal and spatial variation of soil CO2 efflux in a Canadian boreal forest. Soil Biol Biochem. 32:35-45.
Reino S, Rodríguez-Rastrero M, Escolano O, Welte L, Bueno J, Fernández JLL, Schmid T, Millán R. 2014. In Situ Chemical Oxidation Based on Hydrogen Peroxide: Optimization of Its Application to an Hydrocarbon Polluted Site. In: Jiménez E, Cabañas B, Lefebvre G, editors. Environment, Energy and Climate Change I: Environmental Chemistry of Pollutants and Wastes. Vol. 32. Heidelberg: Springer International Publishing. p. 207-228.
Roehm C. 2005. Respiration in Aquatic Ecosystems. In: del Giorgio PJW, editor. Respiration in Aquatic Ecosystems. USA: Oxford University Press. p. 83-102.
Ron EZ, Rosenberg E. 2014. Enhanced bioremediation of oil spills in the sea. Curr Opin Biotechnol. 27:191-194.
Savage KE, Davidson EA. 2003. A comparison of manual and automated systems for soil CO2 flux measurements: trade-offs between spatial and temporal resolution. J Exp Bot. 54:891-899.
Shahi A, Aydin S, Ince B, Ince O. 2016. Evaluation of microbial population and functional genes during the bioremediation of petroleum-contaminated soil as an effective monitoring approach. Ecotoxicol Environ Saf. 125:153-160.
Soil Survey Staff. 2014. Keys to Soil Taxonomy. 12th ed. Washington, DC: USDA-Natural Resources Conservation Service.
Tarazona JV, Fernandez MD, Vega MM. 2005. Regulation of Contaminated Soils in Spain - A New Legal Instrument. J Soils Sediments 5:121-124.
Zeileis A, Hothorn T. 2002. Diagnostic Checking in Regression Relationships. R News 2:7-10.